SEC63 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30222

Novus Biologicals, part of Bio-Techne
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NBP3-30222
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SEC63 Antibodies »

Product Specifications

Immunogen

Between 700 and 750

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SEC63 Antibody

SEC63 Antibody

Immunoprecipitation:SEC63 AntibodyNBP3-30222] -

Immunoprecipitation:SEC63 AntibodyNBP3-30222] -Detection of human SEC63 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SEC63 antibody NBP3-30222 (lot NBP3-30222-1) used for IP at 6 µg per reaction. SEC63 was also immunoprecipitated by rabbit anti-SEC63 antibody BL19995. For blotting immunoprecipitated SEC63, NBP3-30222 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for SEC63 Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SEC63

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER.

Alternate Names

PRO2507, SEC63 homolog (S. cerevisiae), SEC63LERdj2, SEC63-like (S. cerevisiae), translocation protein SEC63 homolog

Gene Symbol

SEC63

Additional SEC63 Products

Product Documents for SEC63 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SEC63 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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