Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SECISBP2 Antibodies »

Product Summary for SECISBP2 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 804 to 854 of human SECIS Binding Protein 2 using the numbering given in entry NP_076982.3 (GeneID 79048).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SECISBP2 Antibody

Western Blot: SECISBP2 Antibody [NBP2-22301]

Western Blot: SECISBP2 Antibody [NBP2-22301]

Western Blot: SECISBP2 Antibody [NBP2-22301] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. NBP2-22301 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: SECISBP2 Antibody [NBP2-22301]

Immunoprecipitation: SECISBP2 Antibody [NBP2-22301]

Immunoprecipitation: SECISBP2 Antibody [NBP2-22301] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22301 used for IP at 6 ug/mg lysate. SBP2 was also immunoprecipitated by rabbit anti-SBP2 antibody. For blotting immunoprecipitated SBP2, NBP2-22301 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for SECISBP2 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:500 - 1:2500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SECISBP2

SECISBP2 is an RNA binding protein that interacts with the Sec insertion sequence (SECIS) element in selenoprotein mRNAs. The incorporation of selenocysteine into a protein requires the concerted action of an mRNA element called a sec insertion sequence (SECIS), a selenocysteine-specific translation elongation factor and a SECIS binding protein. With these elements in place, a UGA codon can be decoded as selenocysteine. Mutations in the SECISBP2 gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism.

Alternate Names

DKFZp686C09169, SBP2SECIS-binding protein 2, SECIS binding protein 2, selenocysteine insertion sequence binding protein 2, selenocysteine insertion sequence-binding protein 2

Gene Symbol

SECISBP2

UniProt

NP_076982.3 (Human)

Additional SECISBP2 Products

Product Documents for SECISBP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SECISBP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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