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Semaphorin 3A Antibody (M317)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23375

Novus Biologicals, part of Bio-Techne
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NBP3-23375

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M317

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Clone M317 was generated from a synthetic peptide (coupled to carrier protein) corresponding to amino acids in the central region of human Semaphorin 3A. The sequence used is highly conserved in rat and mouse Semaphorin 3A, and has low homology to other semaphorin family members. [UniProt# Q14563]

Specificity

The antibody detects a 95kDa protein corresponding to the apparent molecular mass of Semaphorin 3A on SDS-PAGE immunoblots of human recombinant Semaphorin 3A and adult mouse brain lysates.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Semaphorin 3A Antibody (M317)

Semaphorin 3A Antibody (M317)

Western Blot: Semaphorin 3A Antibody (M317) [NBP3-23375] -

Western Blot: Semaphorin 3A Antibody (M317) [NBP3-23375] - Western blots of human recombinant Semaphorin 3A/Fc chimera (95/125 kDa) (lanes 1-4). The blots were probed with rabbit polyclonal Semaphorin 3A (Central region) at 1:250 (lane 1) and 1:1000 (lane 2) and mouse monoclonal Semaphorin 3A (Central region) at 1:250 (lane 3) and 1:1000 (lane 4). Both antibodies recognize the 95 kDa and 125 kDa forms of the recombinant Semaphorin 3A.

Applications for Semaphorin 3A Antibody (M317)

Application
Recommended Usage

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Semaphorin 3A

SEMA3A - sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A

Alternate Names

Sema3A

Gene Symbol

SEMA3A

Additional Semaphorin 3A Products

Product Documents for Semaphorin 3A Antibody (M317)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Semaphorin 3A Antibody (M317)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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