Semaphorin 3A Antibody (M317)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23375
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M317
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Semaphorin 3A Antibody (M317)
Immunogen
Clone M317 was generated from a synthetic peptide (coupled to carrier protein) corresponding to amino acids in the central region of human Semaphorin 3A. The sequence used is highly conserved in rat and mouse Semaphorin 3A, and has low homology to other semaphorin family members. [UniProt# Q14563]
Specificity
The antibody detects a 95kDa protein corresponding to the apparent molecular mass of Semaphorin 3A on SDS-PAGE immunoblots of human recombinant Semaphorin 3A and adult mouse brain lysates.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Semaphorin 3A Antibody (M317)
Western Blot: Semaphorin 3A Antibody (M317) [NBP3-23375] -
Western Blot: Semaphorin 3A Antibody (M317) [NBP3-23375] - Western blots of human recombinant Semaphorin 3A/Fc chimera (95/125 kDa) (lanes 1-4). The blots were probed with rabbit polyclonal Semaphorin 3A (Central region) at 1:250 (lane 1) and 1:1000 (lane 2) and mouse monoclonal Semaphorin 3A (Central region) at 1:250 (lane 3) and 1:1000 (lane 4). Both antibodies recognize the 95 kDa and 125 kDa forms of the recombinant Semaphorin 3A.Applications for Semaphorin 3A Antibody (M317)
Application
Recommended Usage
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Semaphorin 3A
Alternate Names
Sema3A
Gene Symbol
SEMA3A
Additional Semaphorin 3A Products
Product Documents for Semaphorin 3A Antibody (M317)
Product Specific Notices for Semaphorin 3A Antibody (M317)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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