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Semaphorin 3A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23223

Novus Biologicals, part of Bio-Techne
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NBP3-23223

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Semaphorin 3A Antibody

Immunogen

Semaphorin 3A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the central region of human Semaphorin 3A. The sequence used is highly conserved in rat and mouse Semaphorin 3A, and has low homology to other semaphorin family members. [UniProt# Q14563]

Specificity

The antibody detects a 95kDa protein corresponding to the apparent molecular mass of Semaphorin 3A on SDS-PAGE immunoblots of human recombinant Semaphorin 3A and neonatal rat brain lysates.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Semaphorin 3A Antibody

Semaphorin 3A Antibody

Western Blot: Semaphorin 3A Antibody [NBP3-23223] -

Western Blot: Semaphorin 3A Antibody [NBP3-23223] - Western blots of neonatal rat brain (lanes 1, 3 & 5) and human recombinant Semaphorin 3A/Fc chimera (95/125 kDa) (lanes 2, 4 & 6). Blots were probed with anti-Semaphorin 3A (NBP3-23380) (lanes 1 & 2), anti-Semaphorin 3A (NBP3-23223) (lanes 3 & 4) and anti-Semaphorin 3A (NBP3-23379) (lane 5 & 6). The antibodies recognize both the 95 kDa and 125 kDa forms of the recombinant Semaphorin 3A.

Applications for Semaphorin 3A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

6:00

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Semaphorin 3A

SEMA3A - sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A

Alternate Names

Sema3A

Gene Symbol

SEMA3A

Additional Semaphorin 3A Products

Product Documents for Semaphorin 3A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Semaphorin 3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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