Semaphorin 3E Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1433
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KPEHYRLPRHTLDS corresponding to C-Terminus according to NP_036563.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Semaphorin 3E Antibody
![Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-1433]](https://resources.bio-techne.com/images/products/Semaphorin-3E-Antibody-Immunohistochemistry-Paraffin-NB100-1433-img0005.jpg "Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-1433]")
Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-1433]
Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-1433] - Human Adrenal Gland at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry/Immunofluorescence: Goat Polyclonal Semaphorin 3E Antibody [NB100-1433] -
Immunocytochemistry/Immunofluorescence: Goat Polyclonal Semaphorin 3E Antibody [NB100-1433] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, PM and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunocytochemistry/Immunofluorescence: Goat Polyclonal Semaphorin 3E Antibody [NB100-1433] -
Immunocytochemistry/Immunofluorescence: Goat Polyclonal Semaphorin 3E Antibody [NB100-1433] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Applications for Semaphorin 3E Antibody
Application
Recommended Usage
ELISA
Detection limit 1:64000
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
2 - 4 ug/ml
Immunohistochemistry-Paraffin
2 - 4 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Semaphorin 3E
Additional Semaphorin 3E Products
Product Documents for Semaphorin 3E Antibody
Product Specific Notices for Semaphorin 3E Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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