Semaphorin 3E Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-55417
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-NGGRYGTTKDYPDD corresponding to internal region according to NP_036563.1, NP_001171600.1.
Epitope
NGGRYGTTKDYPDD
Specificity
This antibody is expected to recognise both reported isoforms (NP_036563.1; NP_001171600.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Semaphorin 3E Antibody
![Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-55417]](https://resources.bio-techne.com/images/products/Semaphorin-3E-Antibody-Immunohistochemistry-Paraffin-NB100-55417-img0003.jpg "Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-55417]")
Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-55417]
Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-55417] - Staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for Semaphorin 3E Antibody
Application
Recommended Usage
Immunohistochemistry
4 - 6 ug/ml
Immunohistochemistry-Paraffin
1:200
Peptide ELISA
Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave an approx. 35 kDa band in human prostate lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2 kDa band according to NP_036563.1. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human prostate shows membranous staining of secretory cells in the gland.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Semaphorin 3E
Alternate Names
Sema3E, SEMAH
Gene Symbol
SEMA3E
UniProt
Additional Semaphorin 3E Products
Product Documents for Semaphorin 3E Antibody
Product Specific Notices for Semaphorin 3E Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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