Semaphorin 4A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49588

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-49588
NBP2-49588-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Semaphorin 4A Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SGVEYTRLAVETAQGLDGHSHLVMYLGTTTGSLHKAVVSGDSSAHLVEEIQLFPDPEPVRNLQLAPTQGAVFVGFSGGVWRVPRANCSVYE

Reactivity Notes

Mouse (86%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Semaphorin 4A Antibody

Immunocytochemistry/ Immunofluorescence: Semaphorin 4A Antibody [NBP2-49588]

Immunocytochemistry/ Immunofluorescence: Semaphorin 4A Antibody [NBP2-49588]

Immunocytochemistry/Immunofluorescence: Semaphorin 4A Antibody [NBP2-49588] - Staining of human cell line RT4 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Semaphorin 4A Antibody [NBP2-49588]

Immunohistochemistry-Paraffin: Semaphorin 4A Antibody [NBP2-49588]

Immunohistochemistry-Paraffin: Semaphorin 4A Antibody [NBP2-49588] - Staining of human tonsil tissue. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Semaphorin 4A Antibody [NBP2-49588]

Immunohistochemistry-Paraffin: Semaphorin 4A Antibody [NBP2-49588]

Immunohistochemistry-Paraffin: Semaphorin 4A Antibody [NBP2-49588] - Staining of human tonsil shows strong membranous positivity in a subset of non-germinal center cells.

Applications for Semaphorin 4A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP2-49588 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Semaphorin 4A

Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular "Semaphorin" domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. The transmembrane semaphorins are characterized by an additional 80 amino acid transmembrane domain and an 80-110 amino acid cytoplasmic domain. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. Semaphorin 4A (SEMA4A), also designated Semaphorin B, is a type I membrane protein. The SEMA4A gene encoding the protein localizes to chromosome 1q22. SEMA4A provides signals to specify territories inaccessible for growing neurons, inhibiting axonal extension.

Alternate Names

CORD10, SEMA4A, SEMAB, SEMB

Gene Symbol

SEMA4A

Additional Semaphorin 4A Products

Product Documents for Semaphorin 4A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Semaphorin 4A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.