Semaphorin 4B Antibody (4B2)
Novus Biologicals, part of Bio-Techne | Catalog # H00010509-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4B2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SEMA4B (NP_064595, 592 a.a. ~ 689 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GEKPCEQVQFQPNTVNTLACPLLSNLATRLWLRNGAPVNASASCHVLPTGDLLLVGTQQLGEFQCWSLEEGFQQLVASYCPEVVEDGVADQTDEGGSV
Reactivity Notes
Human. Other species not tested.
Specificity
SEMA4B - sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Semaphorin 4B Antibody (4B2)
![Western Blot: Semaphorin 4B Antibody (4B2) [H00010509-M03]](https://resources.bio-techne.com/images/products/Semaphorin-4B-Antibody-4B2-Western-Blot-H00010509-M03-img0002.jpg "Western Blot: Semaphorin 4B Antibody (4B2) [H00010509-M03]")
Western Blot: Semaphorin 4B Antibody (4B2) [H00010509-M03]
Western Blot: Semaphorin 4B Antibody (4B2) [H00010509-M03] - Analysis of SEMA4B expression in transfected 293T cell line by SEMA4B monoclonal antibody (M03), clone 4B2. Lane 1: SEMA4B transfected lysate (Predicted MW: 10.89 KDa). Lane 2: Non-transfected lysate.![ELISA: Semaphorin 4B Antibody (4B2) [H00010509-M03]](https://resources.bio-techne.com/images/products/Semaphorin-4B-Antibody-4B2-ELISA-H00010509-M03-img0001.jpg "ELISA: Semaphorin 4B Antibody (4B2) [H00010509-M03]")
ELISA: Semaphorin 4B Antibody (4B2) [H00010509-M03]
ELISA: Semaphorin 4B Antibody (4B2) [H00010509-M03] - Detection limit for recombinant GST tagged SEMA4B is approximately 0.1ng/ml as a capture antibody.Applications for Semaphorin 4B Antibody (4B2)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Semaphorin 4B
Additional Semaphorin 4B Products
Product Documents for Semaphorin 4B Antibody (4B2)
Product Specific Notices for Semaphorin 4B Antibody (4B2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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