Semaphorin 4D/CD100 Antibody (010)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90666

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-90666-50ul
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 010

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Semaphorin 4D/CD100 Antibodies »

Product Summary for Semaphorin 4D/CD100 Antibody (010)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Semaphorin 4D/CD100 (Accession#: NP_038688.2; Met1-Arg733).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Semaphorin 4D/CD100 Antibody (010)

Flow Cytometry: Semaphorin 4D/CD100 Antibody (010) [NBP2-90666]

Flow Cytometry: Semaphorin 4D/CD100 Antibody (010) [NBP2-90666]

Flow Cytometry: Semaphorin 4D/CD100 Antibody (010) [NBP2-90666] - Flow cytometric analysis of SEMA4D(CD100) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse SEMA4D(CD100), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for Semaphorin 4D/CD100 Antibody (010)

Application
Recommended Usage

Flow Cytometry

1:25-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Semaphorin 4D/CD100

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100, a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Alternate Names

A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ

Gene Symbol

SEMA4D

Additional Semaphorin 4D/CD100 Products

Product Documents for Semaphorin 4D/CD100 Antibody (010)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Semaphorin 4D/CD100 Antibody (010)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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