Semaphorin 4D/CD100 Antibody (21E8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26330

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG4 (S228P) Clone # 21E8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Semaphorin 4D/CD100 Antibodies »

Product Specifications

Immunogen

Recombinant Human Semaphorin 4D/CD100 protein [UniProt Q92854]

Clonality

Monoclonal

Host

Human

Isotype

IgG4 (S228P)

Scientific Data Images for Semaphorin 4D/CD100 Antibody (21E8)

Semaphorin 4D/CD100 Antibody (21E8)

ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] -

ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - The Binding Activity of Mouse Semaphorin 4D/CD100 with Semaphorin 4D/CD100 Antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Semaphorin 4D/CD100 at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody, the EC50 is 98.14-174.8 ng/mL.
Semaphorin 4D/CD100 Antibody (21E8)

ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] -

ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - The Binding Activity of Human Semaphorin 4D/CD100 with Semaphorin 4D/CD100 Antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Semaphorin 4D/CD100 at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody, the EC50 is 1.082-4.805 ng/mL.
Semaphorin 4D/CD100 Antibody (21E8)

Flow Cytometry: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] -

Flow Cytometry: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2ug/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Applications for Semaphorin 4D/CD100 Antibody (21E8)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% Glycerol, 0.01M PBS, PH 7.4

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Semaphorin 4D/CD100

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100, a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Alternate Names

A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ

Gene Symbol

SEMA4D

Additional Semaphorin 4D/CD100 Products

Product Documents for Semaphorin 4D/CD100 Antibody (21E8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Semaphorin 4D/CD100 Antibody (21E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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