Semaphorin 4D/CD100 Antibody (21E8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26330
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG4 (S228P) Clone # 21E8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Semaphorin 4D/CD100 Antibody (21E8)
Immunogen
Recombinant Human Semaphorin 4D/CD100 protein [UniProt Q92854]
Clonality
Monoclonal
Host
Human
Isotype
IgG4 (S228P)
Scientific Data Images for Semaphorin 4D/CD100 Antibody (21E8)
ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] -
ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - The Binding Activity of Mouse Semaphorin 4D/CD100 with Semaphorin 4D/CD100 Antibody.Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Semaphorin 4D/CD100 at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody, the EC50 is 98.14-174.8 ng/mL.
ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] -
ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - The Binding Activity of Human Semaphorin 4D/CD100 with Semaphorin 4D/CD100 Antibody.Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Semaphorin 4D/CD100 at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody, the EC50 is 1.082-4.805 ng/mL.
Flow Cytometry: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] -
Flow Cytometry: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2ug/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.Applications for Semaphorin 4D/CD100 Antibody (21E8)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50% Glycerol, 0.01M PBS, PH 7.4
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Semaphorin 4D/CD100
Alternate Names
A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ
Gene Symbol
SEMA4D
Additional Semaphorin 4D/CD100 Products
Product Documents for Semaphorin 4D/CD100 Antibody (21E8)
Product Specific Notices for Semaphorin 4D/CD100 Antibody (21E8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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