Semaphorin 4D/CD100 Antibody (JE63-21)

Western Blot: Semaphorin 4D/CD100 Antibody (JE63-21) [NBP3-32119]

Western Blot: Semaphorin 4D/CD100 Antibody (JE63-21) [NBP3-32119] - Western blot analysis of Semaphorin 4D/CD100 on different lysates with Rabbit anti-Semaphorin 4D/CD100 antibody (NBP3-32119) at 1/500 dilution.

Lane 1: Jurkat cell lysate
Lane 2: Daudi cell lysate
Lane 3: HL-60 cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 96 kDa
Observed band size: 130 kDa

Exposure time: 30 seconds;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32119) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.