Semaphorin 4D/CD100 Antibody (JE63-21)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32119
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE63-21
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within human Semaphorin 4D/CD100 aa 126-175/862. (Uniprot: Q92854)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Semaphorin 4D/CD100 Antibody (JE63-21)
Western Blot: Semaphorin 4D/CD100 Antibody (JE63-21) [NBP3-32119]
Western Blot: Semaphorin 4D/CD100 Antibody (JE63-21) [NBP3-32119] - Western blot analysis of Semaphorin 4D/CD100 on different lysates with Rabbit anti-Semaphorin 4D/CD100 antibody (NBP3-32119) at 1/500 dilution.Lane 1: Jurkat cell lysate
Lane 2: Daudi cell lysate
Lane 3: HL-60 cell lysate
Lysates/proteins at 10 ug/Lane.
Predicted band size: 96 kDa
Observed band size: 130 kDa
Exposure time: 30 seconds;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32119) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Applications for Semaphorin 4D/CD100 Antibody (JE63-21)
Application
Recommended Usage
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Semaphorin 4D/CD100
Alternate Names
A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ
Gene Symbol
SEMA4D
Additional Semaphorin 4D/CD100 Products
Product Documents for Semaphorin 4D/CD100 Antibody (JE63-21)
Product Specific Notices for Semaphorin 4D/CD100 Antibody (JE63-21)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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