Semaphorin 5A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-41107

Novus Biologicals, part of Bio-Techne
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NB100-41107
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Semaphorin 5A Antibodies »

Product Specifications

Immunogen

Peptide with sequence DEATKKACYSKGKS corresponding to internal region according to NP_003957.1.

Specificity

The immunizing peptide corresponds to a part of the extracellular domain.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Semaphorin 5A Antibody

Immunohistochemistry-Paraffin: Semaphorin 5A Antibody [NB100-41107]

Immunohistochemistry-Paraffin: Semaphorin 5A Antibody [NB100-41107]

Immunohistochemistry-Paraffin: Semaphorin 5A Antibody [NB100-41107] - (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for Semaphorin 5A Antibody

Application
Recommended Usage

Immunohistochemistry

4 - 6 ug/ml

Immunohistochemistry-Paraffin

4 - 6 ug/ml

Peptide ELISA

Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave an approx. 90 kDa band in mouse brain lysates and approx. 25 kDa band in human brain (cerebellum), colon, heart and kidney lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 121 kDa band according to NP_003957.1. The 90 kDa band and 25 kDa band were successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows membanous and vesicular staining in PCT cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Semaphorin 5A

Members of the semaphorin protein family are involved in axonal guidance during neural development. Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with limbic function. The thrombospondin repeats of Semaphorin 5a physically interact with both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans, inducing attractive and inhibitory guidance cues, respectively. The biological responses to Semaphorin 5a are induced through binding to its high-affinity receptor, plexin B3, triggering intracellular signaling through Met.

Alternate Names

SEMA5A, Semaphorin-F

Entrez Gene IDs

9037 (Human); 20356 (Mouse); 310207 (Rat)

Gene Symbol

SEMA5A

UniProt

NP_003957.1 (Human)

Additional Semaphorin 5A Products

Product Documents for Semaphorin 5A Antibody

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Product Specific Notices for Semaphorin 5A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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