Semaphorin 5B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56604

Novus Biologicals, part of Bio-Techne
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NBP2-56604
NBP2-56604-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (99%), Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Semaphorin 5B Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: QESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Semaphorin 5B Antibody

Immunohistochemistry-Paraffin: Semaphorin 5B Antibody [NBP2-56604]

Immunohistochemistry-Paraffin: Semaphorin 5B Antibody [NBP2-56604]

Immunohistochemistry-Paraffin: Semaphorin 5B Antibody [NBP2-56604] - Staining in human cerebral cortex and liver tissues using anti-SEMA5B antibody. Corresponding SEMA5B RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Semaphorin 5B Antibody [NBP2-56604]

Immunocytochemistry/ Immunofluorescence: Semaphorin 5B Antibody [NBP2-56604]

Immunocytochemistry/Immunofluorescence: Semaphorin 5B Antibody [NBP2-56604] - Staining of human cell line SH-SY5Y shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Semaphorin 5B Antibody [NBP2-56604]

Immunohistochemistry-Paraffin: Semaphorin 5B Antibody [NBP2-56604]

Immunohistochemistry-Paraffin: Semaphorin 5B Antibody [NBP2-56604] - Staining of human cerebral cortex shows high expression.
View 1 more images

Applications for Semaphorin 5B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
ICCI/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-P Retrieval method: HIER pH6.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Semaphorin 5B

Members of the semaphorin protein family, such as SEMA5B, are involved in axonal guidance during neural development (Adams et al., 1996 (PubMed 8817451)).(supplied by OMIM)

Alternate Names

SEMA5B, SEMAG, SEMG

Gene Symbol

SEMA5B

Additional Semaphorin 5B Products

Product Documents for Semaphorin 5B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Semaphorin 5B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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