Semaphorin 6A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23387
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken, Xenopus
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Semaphorin 6A Antibody
Immunogen
Semaphorin 6A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the cytoplasmic region of human Sema-6A. The sequence used has high homology to similar regions in rat and mouse Sema-6A, and has low homology to other semaphorin family members. [UniProt# Q9H2E6]
Specificity
The antibody detects a 120kDa protein corresponding to the apparent molecular mass ofSemaphorin 6A on SDS-PAGE immunoblots of Sema-6A transfected COS7 cells, human colorectal cancer cells, and rat PC12 cells. In addition, shRNA knockdown of Sema-6A expression significantly reduces detection of Sema-6A using anti-Sema-6A (a.a. 772-787) antibody.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Applications for Semaphorin 6A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250
Immunoprecipitation
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Semaphorin 6A
Alternate Names
Sema6A
Gene Symbol
SEMA6A
Additional Semaphorin 6A Products
Product Documents for Semaphorin 6A Antibody
Product Specific Notices for Semaphorin 6A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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