Senataxin Antibody (5D8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52727

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse Clone # 5D8

Format

BSA Free

Concentration

1.0 mg/ml

View all Senataxin Antibodies »

Product Summary for Senataxin Antibody (5D8) - BSA Free

Immunogen

Partial recombinant human Senataxin protein (between amino acids 2300-2600) [UniProt Q7Z333]

Clonality

Monoclonal

Host

Mouse

Scientific Data Images for Senataxin Antibody (5D8) - BSA Free

Immunohistochemistry-Paraffin: Senataxin Antibody (5D8) - BSA Free [NBP2-52727]

Immunohistochemistry-Paraffin: Senataxin Antibody (5D8) - BSA Free [NBP2-52727]

Immunohistochemistry-Paraffin: Senataxin Antibody (5D8) [NBP2-52727] - Senataxin antibody clone 5D8 was tested on human prostate tissue sections and generated strong cytoplasmic-nuclear staining in the secretary/basal epithelial cells as well as the dense connective tissue.
Flow Cytometry: Senataxin Antibody (5D8) - BSA Free [NBP2-52727]

Flow Cytometry: Senataxin Antibody (5D8) - BSA Free [NBP2-52727]

Flow Cytometry: Senataxin Antibody (5D8) [NBP2-52727] - An intracellular stain was performed on Jurkat cells with antibody NBP2-52727 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Secondary Antibody.
Flow (Intracellular): Senataxin Antibody (5D8) - BSA Free [NBP2-52727]

Flow (Intracellular): Senataxin Antibody (5D8) - BSA Free [NBP2-52727]

Flow (Intracellular): Senataxin Antibody (5D8) [NBP2-52727] - An intracellular stain was performed on HepG2 cells with antibody NBP2-52727 (blue) and a matched isotype control NBP2-27231 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Applications for Senataxin Antibody (5D8) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

5 ug/mL

Flow Cytometry

5 ug/mL

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Senataxin

Senataxin (SETX, ALS4, AOA2, SCAR1) belongs to the DNA/NAM7 helicase family. The protein encoded for by Senataxin has a DNA/RNA helicase domain at its C-terminal end. Senataxin may play a role in RNA maturation. It may also be involved in DNA double-strand breaks damage response caused by oxidative stress. Cellular localization of Senataxin is limited to the nucleus and cytoplasm. Senataxin is abundantly expressed in skeletal muscle. Expression of Senataxin is also seen in heart, fibroblast, placenta, liver, and weakly in brain and lung. Post translational modifications of Senataxin include acetylation and phosphorylation. There are 3 known isoforms (302.9, 299.4 and 306.3 kDa).

Alternate Names

ALS4recessive, non-Friedreich type 1, AOA2FLJ43459, DKFZp781B151, KIAA0625FLJ12840, senataxin, SETX

Gene Symbol

SETX

UniProt

Q7Z333 (Human)

Additional Senataxin Products

Product Documents for Senataxin Antibody (5D8) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Senataxin Antibody (5D8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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