Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Knockdown Validated, Knockout Validated, Western Blot

Cited:

Chemotaxis, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Senataxin Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2627 and 2677 of human senataxin (NP_055861.2).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

302 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Senataxin Antibody

Western Blot: Senataxin Antibody [NB100-57543]

Western Blot: Senataxin Antibody [NB100-57543]

Western Blot: Senataxin Antibody [NB100-57543] - Detection of Human Senataxin by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody NB100-57543 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Senataxin Antibody [NB100-57543]

Immunoprecipitation: Senataxin Antibody [NB100-57543]

Immunoprecipitation: Senataxin Antibody [NB100-57543] - Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Senataxin antibody NB100-57543 used for IP at 6 ug per reaction. Senataxin was also immunoprecipitated by rabbit anti-Senataxin antibody NB100-57542. For blotting immunoprecipitated Senataxin, NB100-57543 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Senataxin Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID 26700805).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Senataxin

Mutations in Senataxin result in the neurodegenerative disorders Ataxia-Oculomotor Apraxia 2 (AOA2) and amyotrophic lateral sclerosis (ALS4). Senataxin bears an RNA/DNA helicase domain suggesting a role in DNA and RNA processing and has recently been found to be involved in the DNA damage response. Alternate names for Senataxin include SEN1 homolog, SETX, ALS4, KIAA0625, SCAR1, and AOA2.

Alternate Names

ALS4recessive, non-Friedreich type 1, AOA2FLJ43459, DKFZp781B151, KIAA0625FLJ12840, senataxin, SETX

Entrez Gene IDs

23064 (Human)

Gene Symbol

SETX

UniProt

Q7Z333 (Human)

Additional Senataxin Products

Product Documents for Senataxin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Senataxin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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