SENP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56405

Novus Biologicals, part of Bio-Techne
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NB100-56405
NB100-56405SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SENP1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide corresponding to aa 578-590 of human SENP1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SENP1 Antibody

Western Blot: SENP1 Antibody [NB100-56405]

Western Blot: SENP1 Antibody [NB100-56405]

Western Blot: SENP1 Antibody [NB100-56405] - analysis of SENP1 in A) Daudi, B) HeLa and C) NIH 3T3 cell lysate using this antibody.
Simple Western: SENP1 Antibody [NB100-56405]

Simple Western: SENP1 Antibody [NB100-56405]

Simple Western: SENP1 Antibody [NB100-56405] - Simple Western lane view shows a specific band for SENP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for SENP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 27882949)

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 27882949)

Simple Western

1:100

Western Blot

3 - 5 ug/ml
Application Notes
A 66 kDa band is observed. In human and mouse, SENP1 has different isoforms between 664 and 699 amino acids long. Additional bands seen in western blot may represent other SUMOlated proteins. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SENP1

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.

Long Name

SUMO-Specific Peptidase 1

Alternate Names

SuPr-2

Entrez Gene IDs

223870 (Mouse)

Gene Symbol

SENP1

Additional SENP1 Products

Product Documents for SENP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SENP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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