SEPHS2 Antibody (2G9)

Novus Biologicals, part of Bio-Techne | Catalog # H00022928-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2G9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SEPHS2 Antibodies »

Product Specifications

Immunogen

SEPHS2 (NP_036380.2, 61 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GCKVPQEALLKLLAGLTRPDVRPPLGRGLVGGQEEASQEAGLPAGAGPSPTFPALGIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMM

Reactivity Notes

Human. Other species not tested.

Specificity

SEPHS2 - selenophosphate synthetase 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SEPHS2 Antibody (2G9)

Western Blot: SEPHS2 Antibody (2G9) [H00022928-M02]

Western Blot: SEPHS2 Antibody (2G9) [H00022928-M02]

Western Blot: SEPHS2 Antibody (2G9) [H00022928-M02] - SEPHS2 monoclonal antibody (M02), clone 2G9 Analysis of SEPHS2 expression in K-562.
Immunohistochemistry-Paraffin: SEPHS2 Antibody (2G9) [H00022928-M02]

Immunohistochemistry-Paraffin: SEPHS2 Antibody (2G9) [H00022928-M02]

Immunohistochemistry-Paraffin: SEPHS2 Antibody (2G9) [H00022928-M02] - Analysis of monoclonal antibody to SEPHS2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Sandwich ELISA: SEPHS2 Antibody (2G9) [H00022928-M02] - Detection limit for recombinant GST tagged SEPHS2 is approximately 0.03ng/ml as a capture antibody.

Applications for SEPHS2 Antibody (2G9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SEPHS2

This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop. Alternatively spliced transcripts have been identified, but their biological validity has not been determined.

Alternate Names

EC 2.7.9.3, Selenophosphate synthase 2, selenophosphate synthetase 2, SPS2water dikinase 2

Entrez Gene IDs

22928 (Human)

Gene Symbol

SEPHS2

OMIM

606218 (Human)

UniProt

NP_036380 (Human)

Additional SEPHS2 Products

Product Documents for SEPHS2 Antibody (2G9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SEPHS2 Antibody (2G9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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