Septin-1 Antibody (1F12)
Novus Biologicals, part of Bio-Techne | Catalog # H00001731-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1F12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SEPT1 (AAH12161, 1 a.a. ~ 367 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDKEYVGFAALPNQLHRKSVKKGFDFTLMVAGESGLGKSTLINSLFLTNLYEDRQVPEASARLTQTLAIERRGVEIEEGGVKVKLTLVDTPGFGDSVDCSDCWLPVVKFIEEQFEQYLRDESGLNRKNIQDSRVHCCLYFISPFGRGLRPLDVAFLRAVHEKVNIIPVIGKADALMPQETQALKQKIRDQLKEEEIHIYQFPECDSDEDEDFKRQDAEMKESIPFAVVGSCEVVRDGGNRPVRGRRYSWGTVEVENPHHCDFLNLRRMLVQTHLQDLKEVTHDLLYEGYRARRLQSLARPGARDRASRSKLSRQSATEIPLPMLPLADTEKLIREKDEELRRMQEMLEKMQAQMQRSQAQGEQSDAL
Specificity
SEPT1 - septin 1 (1F12)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Septin-1 Antibody (1F12)
Western Blot: Septin-1 Antibody (1F12) [H00001731-M03]
Western Blot: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of SEPT1 expression in transfected 293T cell line by SEPT1 monoclonal antibody (M03), clone 1F12. Lane 1: SEPT1 transfected lysatE (42 KDa). Lane 2: Non-transfected lysate.Immunocytochemistry/ Immunofluorescence: Septin-1 Antibody (1F12) [H00001731-M03]
Immunocytochemistry/Immunofluorescence: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of monoclonal antibody to SEPT1 on HeLa cell. Antibody concentration 10 ug/mlImmunoprecipitation: Septin-1 Antibody (1F12) [H00001731-M03]
Immunoprecipitation: Septin-1 Antibody (1F12) [H00001731-M03] - Analysis of SEPT1 transfected lysate using anti-SEPT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SEPT1 rabbit polyclonal antibody.Applications for Septin-1 Antibody (1F12)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Septin-1
Alternate Names
DIFF6SEP1, differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase), LARP, Peanut-like protein 3, PNUTL3MGC20394, septin 1, septin-1, Serologically defined breast cancer antigen NY-BR-24
Entrez Gene IDs
1731 (Human)
Gene Symbol
SEPT1
UniProt
Additional Septin-1 Products
Product Documents for Septin-1 Antibody (1F12)
Product Specific Notices for Septin-1 Antibody (1F12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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