I'm interested in Septin-12 Antibody (NBP1-91640) for IHC-P. I wonder if 1:500 works well on IHC-P, taking into consideration of dilution factor for ICC and IHC.

Name: Septin-12 Antibody
Brand: Novus Biologicals
SKU: NBP1-91640

Septin-12 is widely expressed in various normal and cancerous tissues, however, its expression levels vary from tissue to tissue. For example, its highest expression levels are seen in lymph node, colon, heart, testes, placenta etc. whereas weak to moderate expression may be seen in liver, brain, bronchus, skin etc. Its expression is negligible in spleen, ovary and breasts, and this is the reason you see a wide range of dilutions being suggested for different applications which also have different levels of sensitivity with respect to detection of a given target. In our validation testing for IHC-P staining of human stomach, this antibody worked well at a dilution of 1:500-1:1000 range, however, we suggest you to optimize the best suitable dilution for your assay from your own pilot testing.

Septin-12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91640

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Septin-12 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IHYENYRVIRLNESHLLPRGPGWVNLAPASPGQLTTPRTFKVCRGAHDDSDDEF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Septin-12 Antibody

Western Blot: Septin-12 Antibody [NBP1-91640]

Western Blot: Septin-12 Antibody [NBP1-91640] - Analysis in control (vector only transfected HEK293T lysate) and LY408232 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/ Immunofluorescence: Septin-12 Antibody [NBP1-91640]

Immunocytochemistry/Immunofluorescence: Septin-12 Antibody [NBP1-91640] - Staining of human cell line U-2 OS shows localization to microtubules. Antibody staining is shown in green.

Applications for Septin-12 Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml

Application Notes

ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Septin-12

Septins, such as SEPT12, are conserved GTP-binding proteins that function as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeleton remodeling, as well as infection, neurodegeneration, and neoplasia (Hall et al., 2005 [PubMed 15915442]).[supplied by OMIM]

Alternate Names

FLJ25410, septin 12, septin-12

Gene Symbol

SEPT12

Additional Septin-12 Products

Product Documents for Septin-12 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Septin-12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Septin-12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91640

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all Septin-12 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for Septin-12 Antibody

Western Blot: Septin-12 Antibody [NBP1-91640]

Immunocytochemistry/ Immunofluorescence: Septin-12 Antibody [NBP1-91640]

Applications for Septin-12 Antibody

Immunocytochemistry/ Immunofluorescence

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Septin-12

Alternate Names

Gene Symbol

Additional Septin-12 Products

Product Documents for Septin-12 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Septin-12 Antibody

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