Septin-6 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51930
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-DEVNAFKQRKTA corresponding to internal region according to NP_665798.1, NP_055944.2, NP_665801.1.
Epitope
C-DEVNAFKQRKTA
Localization
In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre
Specificity
This antibody is expected to recognize the reported isoforms NP_665798.1, NP_055944.2 and NP_665801.1. Reported variants NP_665799.1 and NP_665798.1 represent identical protein.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Septin-6 Antibody
![Western Blot: Septin-6 Antibody [NBP1-51930]](https://resources.bio-techne.com/images/products/Septin-6-Antibody-Western-Blot-NBP1-51930-img0005.jpg "Western Blot: Septin-6 Antibody [NBP1-51930]")
Western Blot: Septin-6 Antibody [NBP1-51930]
Western Blot: Septin-6 Antibody [NBP1-51930] - Staining (1ug/ml) of Human Testis (A), (0.1ug/ml) Duodenum (B), (0.5ug/ml) Kidney (C) and (0.3ug/ml) Tonsil (D) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.![Immunohistochemistry-Paraffin: Septin-6 Antibody [NBP1-51930]](https://resources.bio-techne.com/images/products/Septin-6-Antibody-Immunohistochemistry-Paraffin-NBP1-51930-img0004.jpg "Immunohistochemistry-Paraffin: Septin-6 Antibody [NBP1-51930]")
Immunohistochemistry-Paraffin: Septin-6 Antibody [NBP1-51930]
Immunohistochemistry-Paraffin: Septin-6 Antibody [NBP1-51930] - Staining of paraffin embedded Human Tonsil. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Western Blot: Septin-6 Antibody [NBP1-51930]](https://resources.bio-techne.com/images/products/Septin-6-Antibody-Western-Blot-NBP1-51930-img0006.jpg "Western Blot: Septin-6 Antibody [NBP1-51930]")
Western Blot: Septin-6 Antibody [NBP1-51930]
Western Blot: Septin-6 Antibody [NBP1-51930] - Staining (0.1ug/ml) of Daudi (A), Jurkat (B) and MOLT4 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Applications for Septin-6 Antibody
Application
Recommended Usage
Immunohistochemistry
3 - 5 ug/mL
Immunohistochemistry-Paraffin
3 - 5 ug/mL
Peptide ELISA
Detection limit 1:4000
Western Blot
0.1 - 1 ug/mL
Application Notes
WB: Approx 50 kDa band observed in MOLT4, Daudi, Jurkat, Human Testis, Duodenum, Kidney and Tonsil lysates (calculated MW of 49.7 kDa according to NP_055944.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Septin-6
Alternate Names
KIAA0128SEP2septin 2, MGC16619, MGC20339, SEPT2, septin 6, septin-6
Entrez Gene IDs
23157 (Human)
Gene Symbol
SEPTIN6
UniProt
Additional Septin-6 Products
Product Documents for Septin-6 Antibody
Product Specific Notices for Septin-6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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