Septin-8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15116

Novus Biologicals, part of Bio-Techne
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NBP2-15116
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Septin-8 Antibodies »

Product Summary for Septin-8 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Septin-8. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Septin-8 Antibody

Western Blot: Septin-8 Antibody [NBP2-15116]

Western Blot: Septin-8 Antibody [NBP2-15116]

Western Blot: Septin-8 Antibody [NBP2-15116] - Various tissue extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Septin-8 antibody diluted at 1:4000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Septin-8 Antibody [NBP2-15116]

Immunocytochemistry/ Immunofluorescence: Septin-8 Antibody [NBP2-15116]

Immunocytochemistry/Immunofluorescence: Septin-8 Antibody [NBP2-15116] - Septin-8 antibody detects Septin-8 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Septin-8 protein stained by Septin-8 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Septin-8 Antibody [NBP2-15116]

Immunohistochemistry-Paraffin: Septin-8 Antibody [NBP2-15116]

Immunohistochemistry-Paraffin: Septin-8 Antibody [NBP2-15116] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.
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Applications for Septin-8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Septin-8

SEPT8 is a member of the highly conserved septin family. Septins are 40- to 60-kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking (Blaser et al., 2003 [PubMed 12909369]).[supplied by OMIM]

Alternate Names

KIAA0202SEP2, septin 8, septin-8

Gene Symbol

SEPTIN8

UniProt

Q92599 (Human)

Additional Septin-8 Products

Product Documents for Septin-8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Septin-8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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