Serine racemase Antibody (12B2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26302
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 12B2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Serine racemase Antibody (12B2)
Immunogen
A synthesized peptide derived from Human Serine racemase [UniProt Q9GZT4]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Serine racemase Antibody (12B2)
Western Blot: Serine racemase Antibody (12B2) [NBP3-26302] -
Western Blot: Serine racemase Antibody (12B2) [NBP3-26302] - Positive Western Blot detected in: A549 whole cell lysate, U87 whole cell lysate.All lanes: Serine racemase Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Flow Cytometry: Serine racemase Antibody (12B2) [NBP3-26302] -
Flow Cytometry: Serine racemase Antibody (12B2) [NBP3-26302] - Overlay Peak curve showing HepG2 cells stained with Serine racemase Antibody (12B2) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.Applications for Serine racemase Antibody (12B2)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Serine racemase
Alternate Names
D-serine ammonia-lyase, D-serine dehydratase, EC 4.3.1.17, EC 4.3.1.18, EC 5.1.1.18, ILV1, ISO1, L-serine ammonia-lyase, L-serine dehydratase, serine racemase
Gene Symbol
SRR
Additional Serine racemase Products
Product Documents for Serine racemase Antibody (12B2)
Product Specific Notices for Serine racemase Antibody (12B2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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