Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11618
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378)Azide and BSA Free [NBP3-11618]](https://resources.bio-techne.com/images/products/Serpin-A1-alpha-1-Antitrypsin-Antibody-AAT-1378-Azide-and-BSA-Free-Western-Blot-NBP3-11618-img0002.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378)Azide and BSA Free [NBP3-11618]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # AAT/1378
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human Serpin A1/alpha 1-Antitrypsin protein fragment (Uniprot: P01009)
Localization
Cytoplasmic
Specificity
It recognizes a protein of 54kDa, which is identified antitrypsin (AAT). The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.
Marker
Hepatocellular & Histiocytic Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-48476).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378)Azide and BSA Free [NBP3-11618]
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378) - Azide and BSA Free [NBP3-11618] - Analysis of recombinant Alpha-1-Antitrypsin, Jurkat and A549 Cell Lysate using Alpha-1-Antitrypsin Monoclonal Antibody (AAT/1378).![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378) - Azide and BSA Free [NBP3-11618]](https://resources.bio-techne.com/images/products/Serpin-A1-alpha-1-Antitrypsin-Antibody-AAT-1378-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-11618-img0001.jpg "Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378) - Azide and BSA Free [NBP3-11618]")
Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378) - Azide and BSA Free [NBP3-11618]
Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody (AAT/1378) - Azide and BSA Free [NBP3-11618] - Analysis of human Tonsil stained with Alpha-1-Antitrypsin Monoclonal Antibody (AAT/1378).Applications
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5-1 ug/ml
Western Blot
0.5-1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Serpin A1/alpha 1-Antitrypsin
Alternate Names
A1AT, alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor
Gene Symbol
SERPINA1
Additional Serpin A1/alpha 1-Antitrypsin Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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