Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74095

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9A1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Serpin A1/alpha 1-Antitrypsin Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human SERPINA1 expression vector

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1)Azide and BSA Free [NBP2-74095]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1)Azide and BSA Free [NBP2-74095]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY alpha 1 Antitrypsin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-al
Immunohistochemistry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095]

Immunohistochemistry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095]

Immunohistochemistry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095] - Staining of paraffin-embedded Human pancreas tissue using anti-alpha 1 Antitrypsin mouse monoclonal antibody.
Flow Cytometry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095]

Flow Cytometry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095]

Flow Cytometry: Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free [NBP2-74095] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-alpha 1 Antitrypsin antibody, and then analyzed by flow cytometry.
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Applications for Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:500-1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Serpin A1/alpha 1-Antitrypsin

Alpha 1 Antitrypsin, also known as SERPINA1, is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase. The average level of alpha 1 Antitrypsin in plasma is 1.3 g/l.

Alternate Names

A1AT, alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor

Gene Symbol

SERPINA1

Additional Serpin A1/alpha 1-Antitrypsin Products

Product Documents for Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Serpin A1/alpha 1-Antitrypsin Antibody (OTI9A1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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