Serpin B3/SCCA1 Antibody (2F5)
Novus Biologicals, part of Bio-Techne | Catalog # H00006317-M01
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunoprecipitation, Protein Array, Western Blot
Cited:
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2F5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SERPINB3 (NP_008850, 276 a.a. ~ 390 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RETRVDLHLPRFKVEESYDLKDTLRTMGMVDIFNGDADLSGMTGSRGLVLSGVLHKAFVEVTEEGAEAAAATAVVGFGSSPTSTNEEFHCNHPFLFFIRQNKTNSILFYGRFSSP
Specificity
SERPINB3 - serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Serpin B3/SCCA1 Antibody (2F5)
![Western Blot: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01]](https://resources.bio-techne.com/images/products/Serpin-B3-SCCA1-Antibody-2F5-Western-Blot-H00006317-M01-img0004.jpg "Western Blot: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01]")
Western Blot: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01]
Western Blot: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01] - Analysis of SERPINB3 expression in transfected 293T cell line by SERPINB3 monoclonal antibody (M01), clone 2F5.Lane 1: SERPINB3 transfected lysate(44.6 KDa).Lane 2: Non-transfected lysate.![Immunoprecipitation: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01]](https://resources.bio-techne.com/images/products/Serpin-B3-SCCA1-Antibody-2F5-Immunoprecipitation-H00006317-M01-img0002.jpg "Immunoprecipitation: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01]")
Immunoprecipitation: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01]
Immunoprecipitation: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01] - Analysis of SERPINB3 transfected lysate using anti-SERPINB3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SERPINB3 MaxPab rabbit polyclonal antibody.![ELISA: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01]](https://resources.bio-techne.com/images/products/Serpin-B3-SCCA1-Antibody-2F5-Sandwich-ELISA-H00006317-M01-img0003.jpg "ELISA: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01]")
ELISA: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01]
ELISA: Serpin B3/SCCA1 Antibody (2F5) [H00006317-M01] - Detection limit for recombinant GST tagged SERPINB3 is approximately 0.3ng/ml as a capture antibody.Applications for Serpin B3/SCCA1 Antibody (2F5)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Protein Array
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Serpin B3/SCCA1
Alternate Names
SCCA1, T4-A
Entrez Gene IDs
6317 (Human)
Gene Symbol
SERPINB3
OMIM
600517 (Human)
UniProt
Additional Serpin B3/SCCA1 Products
Product Documents for Serpin B3/SCCA1 Antibody (2F5)
Product Specific Notices for Serpin B3/SCCA1 Antibody (2F5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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