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Key Product Details

Species Reactivity

Validated:

Human, C. elegans

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1A12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Summary for Serpin B3/SCCA1 Antibody (OTI1A12) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human SERPINB3(NP_008850) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

44.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Serpin B3/SCCA1 Antibody (OTI1A12) - Azide and BSA Free

Western Blot: Serpin B3/SCCA1 Antibody (OTI1A12)Azide and BSA Free [NBP2-74109]

Western Blot: Serpin B3/SCCA1 Antibody (OTI1A12)Azide and BSA Free [NBP2-74109]

Western Blot: Serpin B3/SCCA1 Antibody (OTI1A12) - Azide and BSA Free [NBP2-74109] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB3 (RC202683, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB3. Positive lysates LY416321 (100ug).
Immunohistochemistry-Paraffin: Serpin B3/SCCA1 Antibody (OTI1A12) - Azide and BSA Free [NBP2-74109]

Immunohistochemistry-Paraffin: Serpin B3/SCCA1 Antibody (OTI1A12) - Azide and BSA Free [NBP2-74109]

Immunohistochemistry-Paraffin: Serpin B3/SCCA1 Antibody (OTI1A12) - Azide and BSA Free [NBP2-74109] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120 degrees C for 3min.
Immunohistochemistry-Paraffin: Serpin B3/SCCA1 Antibody (OTI1A12) - Azide and BSA Free [NBP2-74109]

Immunohistochemistry-Paraffin: Serpin B3/SCCA1 Antibody (OTI1A12) - Azide and BSA Free [NBP2-74109]

Immunohistochemistry-Paraffin: Serpin B3/SCCA1 Antibody (OTI1A12) - Azide and BSA Free [NBP2-74109] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SERPINB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120 degrees C for 3min.

Applications for Serpin B3/SCCA1 Antibody (OTI1A12) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Serpin B3/SCCA1

SerpinB3 may act as a protease inhibitor to modulate the host immune response against tumor cells

Alternate Names

SCCA1, T4-A

Gene Symbol

SERPINB3

Additional Serpin B3/SCCA1 Products

Product Documents for Serpin B3/SCCA1 Antibody (OTI1A12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Serpin B3/SCCA1 Antibody (OTI1A12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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