Serpin D1/Heparin Cofactor II Antibody (25)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90429
Recombinant Monoclonal Antibody
![Western Blot: Serpin D1/Heparin Cofactor II Antibody (25) [NBP2-90429]](https://resources.bio-techne.com/images/products/Serpin-D1-Heparin-Cofactor-II-Antibody-25-Western-Blot-NBP2-90429-img0006.jpg "Western Blot: Serpin D1/Heparin Cofactor II Antibody (25) [NBP2-90429]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 25
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Serpin D1/Heparin Cofactor II (Accession#: NP_032249.3; Met 1-Ser 478).
Specificity
No cross-reactivity in ELISA with: Human SerpinA1 / A1ATHuman SerpinA3 / AACTHuman SerpinA5 / PROCIMouse SerpinF2 / AAP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Serpin D1/Heparin Cofactor II Antibody (25)
Western Blot: Serpin D1/Heparin Cofactor II Antibody (25) [NBP2-90429]
Western Blot: Serpin D1/Heparin Cofactor II Antibody (25) [NBP2-90429] - Anti-Serpin D1/Heparin Cofactor II rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 53 kDa![Immunoprecipitation: Serpin D1/Heparin Cofactor II Antibody (25) [NBP2-90429]](https://resources.bio-techne.com/images/products/Serpin-D1-Heparin-Cofactor-II-Antibody-25-Immunoprecipitation-NBP2-90429-img0004.jpg "Immunoprecipitation: Serpin D1/Heparin Cofactor II Antibody (25) [NBP2-90429]")
Immunoprecipitation: Serpin D1/Heparin Cofactor II Antibody (25) [NBP2-90429]
Immunoprecipitation: Serpin D1/Heparin Cofactor II Antibody (25) [NBP2-90429] - Mouse Serpin D1/Heparin Cofactor II was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 0.5 ul anti-Mouse Serpin D1/Heparin Cofactor II rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Serpin D1/Heparin Cofactor II rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDaApplications for Serpin D1/Heparin Cofactor II Antibody (25)
Application
Recommended Usage
ELISA
1:25000-1:50000
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Serpin D1/Heparin Cofactor II
Alternate Names
HC2, HCF2, Heparin Cofactor II, LS2
Gene Symbol
SERPIND1
Additional Serpin D1/Heparin Cofactor II Products
Product Documents for Serpin D1/Heparin Cofactor II Antibody (25)
Product Specific Notices for Serpin D1/Heparin Cofactor II Antibody (25)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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