Serpin I1/Neuroserpin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1529
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-TMNTSGHDFEEL corresponding to C-Terminus according to NP_005016.1.
Epitope
C Terminus
Specificity
Reported variants represent identical protein (NP_005016.1; NP_001116224.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Serpin I1/Neuroserpin Antibody
Western Blot: Serpin I1/Neuroserpin Antibody [NB100-1529] -
Western Blot: Serpin I1/Neuroserpin Antibody [NB100-1529] - (1ug/ml) staining of Kelly (A) and K562 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Flow Cytometry: Serpin I1/Neuroserpin Antibody [NB100-1529] -
Flow Cytometry: Serpin I1/Neuroserpin Antibody [NB100-1529] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: Serpin I1/Neuroserpin Antibody [NB100-1529] -
Western Blot: Serpin I1/Neuroserpin Antibody [NB100-1529] - (1ug/ml) staining of Human Placenta (A), Mouse Brain (B) and (0.5ug/ml) Rat Braun (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Applications for Serpin I1/Neuroserpin Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
0.5-2 ug/ml
Application Notes
Western blot: Approx 48kDa band observed in lysates of cell lines Kelly and K562 and in Human Placenta and Mouse and Rat Brain lysates (calculated MW of 46.4kDa according to Human NP_005016.1, Mouse NP_033276.1 and Rat NP_446231.1). Primary incubation 1 hour at room temperature.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Serpin I1
Additional Serpin I1 Products
Product Documents for Serpin I1/Neuroserpin Antibody
Product Specific Notices for Serpin I1/Neuroserpin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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