SerpinB2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33188
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human SerpinB2. The exact sequence is proprietary.
Reactivity Notes
Rabbit (83%), Sheep (81%), Bovine (82%).
Localization
Cytoplasm, Secreted, extracellular space
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SerpinB2 Antibody
Western Blot: SerpinB2 Antibody [NBP1-33188]
Western Blot: SerpinB2 Antibody [NBP1-33188] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: SerpinB2 Antibody [NBP1-33188]
Immunocytochemistry/Immunofluorescence: SerpinB2 Antibody [NBP1-33188] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAI2 protein stained by PAI2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.Immunohistochemistry-Paraffin: SerpinB2 Antibody [NBP1-33188]
Immunohistochemistry-Paraffin: SerpinB2 Antibody [NBP1-33188] - NCIN87 xenograft , using PAI-2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for SerpinB2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Serpin B2
Alternate Names
PAI2, PLANH2, Urokinase Inhibitor
Gene Symbol
SERPINB2
Additional Serpin B2 Products
Product Specific Notices for SerpinB2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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