SerpinB2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77964

Novus Biologicals, part of Bio-Techne
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NBP1-77964
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Serpin B2 Antibodies »

Product Summary for SerpinB2 Antibody

Immunogen

This affinity purified SerpinB2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal area of human SerpinB2 protein. (Uniprot: P05120)

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with SerpinB2 proteins from several primates based on 94% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Specificity

This affinity-purified antibody is directed against human SerpinB2 protein. Reactivity occurs against human SerpinB2 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This affinity-purified antibody is directed against human PAI-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for SerpinB2 Antibody

Western Blot: SerpinB2 Antibody [NBP1-77964]

Western Blot: SerpinB2 Antibody [NBP1-77964]

Western Blot: SerpinB2 Antibody [NBP1-77964] - 2 in 50 ug of HeLa whole cell lysates. The band at ~42 kDa (arrowhead) corresponds to SerpinB2 . Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4C. The membrane was washed and reacted with a 1:5,000 dilution of HRP- conjugated Gt-a-Rabbit IgG for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers.
Immunohistochemistry: SerpinB2 Antibody [NBP1-77964]

Immunohistochemistry: SerpinB2 Antibody [NBP1-77964]

Immunohistochemistry: SerpinB2 Antibody [NBP1-77964] - Tissue: heart. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-Plasminogen Activator 2 at 10 ug/mL for 1 h at RT. Staining: Plasminogen Activator II as precipitated red signal with hematoxylin purple nuclear counterstain.

Applications for SerpinB2 Antibody

Application
Recommended Usage

ELISA

1:100000-150000

Immunohistochemistry

10ug/ml

Western Blot

1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA, Immunochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band 42-46.6 kDa in size corresponding to PAI-2 protein by western blotting in the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Serpin B2

Extracellular plasminogen activator inhibitor type-2 (also known as PAI-2, Placental plasminogen activator inhibitor; Monocyte Arg-serpin; Urokinase inhibitor; SERPINB2 serpin peptidase inhibitor, clade B (ovalbumin), member 2) is a coagulation factor and a potent inhibitor of urokinase-type plasminogen activator (u-PA) and also acts as a multifunctional protein. It is present in most cells, especially monocytes and macrophages. PAI-2 exists in two forms, a 60-kDa, secreted, extracellular, glycosylated form and a 43-kDa intracellular form. It is a multifunctional protein that plays a role in cell differentiation, in prevention of programmed cell death, in the regulation of cell proliferation, in the inhibition of microbial proteinases and in the protection against stromal degradation. High levels of the PAI-2 protein are associated with a good prognosis in breast cancer, small cell lung, ovarian cancer, and inhibition of metastasis. PAI-2 also plays a role in inflammation on the surface of the eye. PAI-2 may cooperate with pRb2/p130 in modulating PAI-2 gene expression by chromatin remodeling.

Alternate Names

PAI2, PLANH2, Urokinase Inhibitor

Gene Symbol

SERPINB2

UniProt

P05120, NP_002566.1 (Human)

Additional Serpin B2 Products

Product Documents for SerpinB2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SerpinB2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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