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Serum Response Factor SRF Antibody (PCRP-SRF-1F1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14187

Novus Biologicals, part of Bio-Techne
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NBP3-14187

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-SRF-1F1

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary

Immunogen

Recombinant full-length human Serum Response Factor SRF protein (Uniprot: P11831)

Localization

Nucleus, Nucleoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13784).

Antibody without azide - store at -20 to -80C. Antibody with azide - store at 2 to 8C. Non-hazardous.

Theoretical molecular weight: 40-67kDa

Scientific Data Images

Protein Array: Serum Response Factor SRF Antibody (PCRP-SRF-1F1) - Azide and BSA Free [NBP3-14187]

Protein Array: Serum Response Factor SRF Antibody (PCRP-SRF-1F1) - Azide and BSA Free [NBP3-14187]

Protein Array: Serum Response Factor SRF Antibody (PCRP-SRF-1F1) - Azide and BSA Free [NBP3-14187] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Serum Response Factor SRF Antibody (PCRP-SRF-1F1).

Applications

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Serum Response Factor SRF

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is a cardiac-enriched transcription factor that is required for the appearance of beating sarcomeres in the heart. In addition, SRF may also direct the expression of microRNAs that inhibit the expression of cardiac regulatory factors. SRF binding sites are also constitutive promotor elements in many muscle specific promoters. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk 1 and SRF accessory protein 1 (SAP 1) have DNA binding properties identical to that of p62TCF

Long Name

Serum response factor

Alternate Names

SRF

Gene Symbol

SRF

Additional Serum Response Factor SRF Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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