Serum Response Factor SRF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51976

Novus Biologicals, part of Bio-Techne
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NBP1-51976
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Serum Response Factor SRF Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QASPSRDSSTDLTQ corresponding to internal region according to NP_003122.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Serum Response Factor SRF Antibody

Western Blot: Serum Response Factor SRF Antibody [NBP1-51976]

Western Blot: Serum Response Factor SRF Antibody [NBP1-51976]

Western Blot: Serum Response Factor SRF Antibody [NBP1-51976] - Analysis of untreated (first lane) and TGF-bl-treated (second lane) primary cultured Human Lung fibroblast lysate. Primary incubated overnight at 4C. Detected by western blot using chemiluminescence. Data kindly provided by Nickolai Dulin, Dept Medicine/Pulmonary, Chicago, USA

Applications for Serum Response Factor SRF Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 67 kDa band observed in primary cultured human lung fibroblasts lysates after 24 h treatment with 1ng/ml TGF-bl (PMID: 11893590).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Serum Response Factor SRF

This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).

Long Name

Serum response factor

Alternate Names

SRF

Entrez Gene IDs

6722 (Human); 20807 (Mouse)

Gene Symbol

SRF

UniProt

P11831 (Human)

Additional Serum Response Factor SRF Products

Product Documents for Serum Response Factor SRF Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Serum Response Factor SRF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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