SESN1 Antibody (7I5Y4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15916
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7I5Y4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SESN1 (Q9Y6P5). KTQHYLLQMDGPLPLHYRHYIGIMAAARHQCSYLVNLHVNDFLHVGGDPKWLNGLENAPQKLQNLGELNKVLAHRPWLITKEHIEGLLKAEEHSWSLAELV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SESN1 Antibody (7I5Y4)
Western Blot: SESN1 Antibody (7I5Y4) [NBP3-15916]
Western Blot: SESN1 Antibody (7I5Y4) [NBP3-15916] - Western blot analysis of extracts of various cell lines, using SESN1 Rabbit mAb (NBP3-15916) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Immunohistochemistry-Paraffin: SESN1 Antibody (7I5Y4) [NBP3-15916]
Immunohistochemistry-Paraffin: SESN1 Antibody (7I5Y4) [NBP3-15916] - Immunohistochemistry of paraffin-embedded mouse heart using SESN1 Rabbit mAb (NBP3-15916) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Immunohistochemistry-Paraffin: SESN1 Antibody (7I5Y4) [NBP3-15916]
Immunohistochemistry-Paraffin: SESN1 Antibody (7I5Y4) [NBP3-15916] - Immunohistochemistry of paraffin-embedded human colon carcinoma using SESN1 Rabbit mAb (NBP3-15916) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for SESN1 Antibody (7I5Y4)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SESN1
Alternate Names
MGC138241, MGC142129, p53-regulated protein PA26, PA26p53 activated gene 26, SEST1p53 regulated PA26 nuclear protein, sestrin 1, sestrin-1
Gene Symbol
SESN1
Additional SESN1 Products
Product Documents for SESN1 Antibody (7I5Y4)
Product Specific Notices for SESN1 Antibody (7I5Y4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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