SESN2 Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44990

Novus Biologicals, part of Bio-Techne
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NBP1-44990
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SESN2 Antibodies »

Product Summary for SESN2 Antibody [FITC]

Immunogen

Synthetic peptide taken within amino acid region 1-80 on SESN2 protein.

Localization

Cytoplasm, Nucleus

Specificity

This antibody is specific to SESN2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for SESN2 Antibody [FITC]

Application
Recommended Usage

ELISA

< 1:10000

Western Blot

>1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SESN2

Sestrin 2(SESN2), located at human chromosome 1p35.3, belongs to the sestrin family of proteins. These proteins are cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense (1). This family of proteins is only present in multicellular organisms ranging from nematodes to mammals. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes (2). Peroxiredoxins are the enzymes that metabolize peroxides. Expression of these proteins is regulated by p53, a tumor suppressor protein (3). Recently it was reported that sestrin 2 is not a sulfinic peroxiredoxin reductase (4). Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy (5). Depletion of sestrin 2 in human cancer cells reduced the level of autophagy when autophagy was induced in these cells (5). Similar to sestrin 1, sestrin 2 negatively regulates the activity of mammalian target of rapamycin (mTOR), a positive regulator of cell growth (6). Sestrin 2 may be a promising target in the treatment of chronic obstructive pulmonary disease (COPD). It was reported that mutational inactivation of sestrin 2 induced TGF-Beta signaling and partially reduced the emphysema phenotype in mice model of COPD (7). Sestrin 2 might also be a key target in the treatment of cancer. It was shown to activate AMPK and repress mTORC1 signaling. AMPK is an essential mediator of LKB1 tumor suppressor and it is down-regulated in cancer cells (8). Sestrin 2 is approximately a 57.6kDa protein based on the amino acid predicted MW of 57kDa (480 amino acids).

Alternate Names

DKFZp761M0212, DKFZp761M02121, Hi95, HI95sestrin-2, SES2, SEST2hypoxia induced gene 95, sestrin 2

Entrez Gene IDs

83667 (Human)

Gene Symbol

SESN2

UniProt

P58004 (Human)

Additional SESN2 Products

Product Documents for SESN2 Antibody [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SESN2 Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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