SESN3 Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44986

Novus Biologicals, part of Bio-Techne
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NBP1-44986
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SESN3 Antibodies »

Product Summary for SESN3 Antibody [FITC]

Immunogen

Synthetic peptides corresponding to unique epitope on SESN3. The SESN3 peptide was selected from N-terminal, amino acids 1-50. This peptide was covalently modified post-synthetically to achieve desired antigenicity.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for SESN3 Antibody [FITC]

Application
Recommended Usage

ELISA

< 1:10000

Western Blot

>1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SESN3

Sestrin 3(SESN3), located at human chromosome 11q21, belongs to the sestrin family of proteins. These proteins are cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense (1). This family of proteins is only present in multicellular organisms ranging from nematodes to mammals. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes (2). Peroxiredoxins are the enzymes that metabolize peroxides. Expression of these proteins is regulated by p53, a tumor suppressor protein (3). Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity (4). Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity (5). AKT is a protein kinase that is hyperactivated in cancer. This hyperactivity leads to an increase in intracellular ROS mainly by inhibiting the expression of ROS scavengers downstream of FoxO, such as sestrin 3 (4). This phenomenon was supported by the fact that AKT deficient cells contained high levels of sestrin 3 leading to low levels of ROS. Furthermore, downregulation of sestrin 3 restored ROS levels in these cells (5). Hyperactivation of AKT has been shown to promote uncontrolled cell growth and also protect cells from apoptosis (6). Inhibition of mRNAs from sestrin 3 as well as from sestrin 1 has also been shown to substantially increase intracellular ROS levels. This increase in ROS is linked to Ras-induced mutagenesis which is one of the most common events in cancer (7). Sestrin 3 is approximately a 38.5kDa protein (480 amino acids) in human and 59kDa protein (492 amino acids) in rat and mouse.

Alternate Names

SEST3MGC29667, sestrin 3, sestrin-3

Entrez Gene IDs

143686 (Human)

Gene Symbol

SESN3

UniProt

P58005 (Human)

Additional SESN3 Products

Product Documents for SESN3 Antibody [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SESN3 Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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