SET1B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18682
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SET1B Antibody
Immunogen
Polyclonal antibody raised in rabbit against mouse SET1B (Set domain containing 1B), using a 3 KLH-conjugated synthetic peptides containing sequences from the central part of the protein (1).
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for SET1B Antibody
Western Blot: SET1B Antibody [NBP3-18682]
Western Blot: SET1B Antibody [NBP3-18682] - Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse SET1B , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.ELISA: SET1B Antibody [NBP3-18682]
ELISA: SET1B Antibody [NBP3-18682] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse SET1B . The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,400.Applications for SET1B Antibody
Application
Recommended Usage
ELISA
1:100-1:500
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:20-1:50
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Whole Antiserum
Formulation
Whole antiserum
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: SET1B
Alternate Names
EC 2.1.1, FLJ20803, histone-lysine N-methyltransferase SETD1B, hSET1B, KIAA1076EC 2.1.1.43, KMT2GSET1B, Lysine N-methyltransferase 2G, SET domain containing 1B, SET domain-containing protein 1B, Set1B
Gene Symbol
SETD1B
UniProt
Additional SET1B Products
Product Specific Notices for SET1B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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