SETD1A Antibody (PH00-02)

Western Blot: SETD1A Antibody (PH00-02) [NBP3-32443]

Western Blot: SETD1A Antibody (PH00-02) [NBP3-32443] - Western blot analysis of SETD1A on 293T cell lysates with Rabbit anti-SETD1A antibody (NBP3-32443) at 1/1,000 dilution.

Lysates/proteins at 10 ug/Lane.

Predicted band size: 186 kDa
Observed band size: 300 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32443) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: SETD1A Antibody (PH00-02) [NBP3-32443]

Flow Cytometry: SETD1A Antibody (PH00-02) [NBP3-32443] - Flow cytometric analysis of 293T cells labeling SETD1A.

Cells were fixed and permeabilized.Then stained with the primary antibody (NBP3-32443, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).