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SETD1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12907

Novus Biologicals, part of Bio-Techne
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NBP3-12907

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for SETD1A Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SETD1A. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

186 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SETD1A Antibody

Western Blot: SETD1A Antibody [NBP3-12907]

Western Blot: SETD1A Antibody [NBP3-12907]

Western Blot: SETD1A Antibody [NBP3-12907] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETD1A antibody (NBP3-12907) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SETD1A Antibody [NBP3-12907]

Immunocytochemistry/ Immunofluorescence: SETD1A Antibody [NBP3-12907]

Immunocytochemistry/Immunofluorescence: SETD1A Antibody [NBP3-12907] - SETD1A antibody detects SETD1A protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETD1A protein stained by SETD1A antibody (NBP3-12907) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: SETD1A Antibody [NBP3-12907]

Immunohistochemistry-Paraffin: SETD1A Antibody [NBP3-12907]

Immunohistochemistry-Paraffin: SETD1A Antibody [NBP3-12907] - Mouse brain. SETD1A antibody (NBP3-12907) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for SETD1A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SETD1A

SET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]). Also see SET1B (MIM 611055).[supplied by OMIM]

Alternate Names

histone-lysine N-methyltransferase SETD1A, hSET1A, KIAA0339EC 2.1.1.43, KMT2FSET1, Lysine N-methyltransferase 2F, SET domain containing 1A, SET domain-containing protein 1A, Set1, Set1/Ash2 histone methyltransferase complex subunit SET1, SET1A

Gene Symbol

SETD1A

Additional SETD1A Products

Product Documents for SETD1A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SETD1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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