SETD1A Antibody

Western Blot: SETD1A Antibody [NBP3-18681]

Western Blot: SETD1A Antibody [NBP3-18681] - Figure 2. Western blot analysis using the antibody directed against mouse SETD1A (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse SETD1A , diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 186 kDa) is indicated on the right.

ELISA: SETD1A Antibody [NBP3-18681]

ELISA: SETD1A Antibody [NBP3-18681] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse SETD1A . The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,500.