SETD7 Antibody (OTI2D10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74145

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2D10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SETD7 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SETD7(NP_085151) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

40.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SETD7 Antibody (OTI2D10) - Azide and BSA Free

Western Blot: SETD7 Antibody (OTI2D10)Azide and BSA Free [NBP2-74145]

Western Blot: SETD7 Antibody (OTI2D10)Azide and BSA Free [NBP2-74145]

Western Blot: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD7.
Immunocytochemistry/ Immunofluorescence: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145]

Immunocytochemistry/ Immunofluorescence: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145]

Immunocytochemistry/Immunofluorescence: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SETD7.
Immunohistochemistry: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145]

Immunohistochemistry: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145]

Immunohistochemistry: SETD7 Antibody (OTI2D10) - Azide and BSA Free [NBP2-74145] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SETD7 mouse monoclonal antibody.
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Applications for SETD7 Antibody (OTI2D10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SETD7

SET7 is a member of the SET (SuVar3-9, enhancer of Zeste, Trithorax) domain-containing protein family. SET7 functions as a histone methyltransferase that specifically monomethylates lysine-4 of histone H3. SET7 has also been observed to monomethylate non-histone proteins such as p53, TAF10, and the estrogen receptor. The methylating activity of SET7 is essential to cell cycle progression and genomic stability.

Long Name

Histone-lysine N-methyltransferase SETD7

Alternate Names

EC 2.1.1.364, H3-K4-HMTase SETD7, KIAA1717, KMT7, SET7, SET7/9, SET9

Gene Symbol

SETD7

Additional SETD7 Products

Product Documents for SETD7 Antibody (OTI2D10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SETD7 Antibody (OTI2D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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