SETDB1 Antibody (4A3)

Novus Biologicals, part of Bio-Techne | Catalog # H00009869-M07

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4A3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SETDB1 Antibodies »

Product Specifications

Immunogen

SETDB1 (NP_036564, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSLPGCIGLDAATATVESEEIAELQQAVVEELGISMEELRHFIDEELEKMDCVQQRKKQLAELETWVIQKESEVAHVDQLFDDASRAVTNCESLVKDFY

Specificity

SETDB1 - SET domain, bifurcated 1 (4A3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SETDB1 Antibody (4A3)

Western Blot: SETDB1 Antibody (4A3) [H00009869-M07]

Western Blot: SETDB1 Antibody (4A3) [H00009869-M07]

Western Blot: SETDB1 Antibody (4A3) [H00009869-M07] - Analysis of SETDB1 expression in SW-13 (Cat # L005V1).
Immunocytochemistry/ Immunofluorescence: SETDB1 Antibody (4A3) [H00009869-M07]

Immunocytochemistry/ Immunofluorescence: SETDB1 Antibody (4A3) [H00009869-M07]

Immunocytochemistry/Immunofluorescence: SETDB1 Antibody (4A3) [H00009869-M07] - Analysis of monoclonal antibody to SETDB1 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: SETDB1 Antibody (4A3) [H00009869-M07] - Detection limit for recombinant GST tagged SETDB1 is approximately 0.1ng/ml as a capture antibody.

Applications for SETDB1 Antibody (4A3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SETDB1

The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.[supplied by OMIM]

Alternate Names

ERG-associated protein with SET domain, ESETEC 2.1.1.43, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase SETDB1, histone-lysine N-methyltransferase, H3lysine-9 specific 4, KG1T, KIAA0067H3-K9-HMTase4, KMT1EERG-associated protein with a SET domain, ESET, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1

Entrez Gene IDs

9869 (Human)

Gene Symbol

SETDB1

UniProt

NP_036564 (Human)

Additional SETDB1 Products

Product Documents for SETDB1 Antibody (4A3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SETDB1 Antibody (4A3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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