SETDB1 Antibody (5H6A12) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51677
Key Product Details
Species Reactivity
Human, Mouse, Monkey
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5H6A12
Format
BSA Free
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Purified recombinant fragment of human SETDB1 expressed in E. Coli.
Reactivity Notes
Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SETDB1 Antibody (5H6A12) - BSA Free
![Western Blot: SETDB1 Antibody (5H6A12)BSA Free [NBP1-51677]](https://resources.bio-techne.com/images/products/SETDB1-Antibody-5H6A12-Western-Blot-NBP1-51677-img0007.jpg "Western Blot: SETDB1 Antibody (5H6A12)BSA Free [NBP1-51677]")
Western Blot: SETDB1 Antibody (5H6A12)BSA Free [NBP1-51677]
Western Blot: SETDB1 Antibody (5H6A12) [NBP1-51677] - Analysis using SETDB1 mouse mAb against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5), F9 (6), RAW246.7 (7) and Cos7 (8) cell lysate.![Immunocytochemistry/ Immunofluorescence: SETDB1 Antibody (5H6A12) - BSA Free [NBP1-51677]](https://resources.bio-techne.com/images/products/SETDB1-Antibody-5H6A12-Immunocytochemistry-Immunofluorescence-NBP1-51677-img0005.jpg "Immunocytochemistry/ Immunofluorescence: SETDB1 Antibody (5H6A12) - BSA Free [NBP1-51677]")
Immunocytochemistry/ Immunofluorescence: SETDB1 Antibody (5H6A12) - BSA Free [NBP1-51677]
Immunocytochemistry/Immunofluorescence: SETDB1 Antibody (5H6A12) [NBP1-51677] - Analysis of LOVO cells using SETDB1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Applications for SETDB1 Antibody (5H6A12) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Format
BSA Free
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SETDB1
Alternate Names
ERG-associated protein with SET domain, ESETEC 2.1.1.43, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase SETDB1, histone-lysine N-methyltransferase, H3lysine-9 specific 4, KG1T, KIAA0067H3-K9-HMTase4, KMT1EERG-associated protein with a SET domain, ESET, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1
Entrez Gene IDs
9869 (Human)
Gene Symbol
SETDB1
UniProt
Additional SETDB1 Products
Product Documents for SETDB1 Antibody (5H6A12) - BSA Free
Product Specific Notices for SETDB1 Antibody (5H6A12) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...