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SETDB1 Antibody (HL1645) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25679

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25679

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1645

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SETDB1 The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SETDB1 Antibody (HL1645) - Azide and BSA Free

SETDB1 Antibody (HL1645) - Azide and BSA Free

Western Blot: SETDB1 Antibody (HL1645) - Azide and BSA Free [NBP3-25679] -

Western Blot: SETDB1 Antibody (HL1645) - Azide and BSA Free [NBP3-25679] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL1645] (NBP3-25679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SETDB1 Antibody (HL1645) - Azide and BSA Free

Western Blot: SETDB1 Antibody (HL1645) - Azide and BSA Free [NBP3-25679] -

Western Blot: SETDB1 Antibody (HL1645) - Azide and BSA Free [NBP3-25679] - HeLa whole cell and nuclear extract (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with SETDB1 antibody [HL1645] (NBP3-25679) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SETDB1 Antibody (HL1645) - Azide and BSA Free

Immunohistochemistry-Paraffin: SETDB1 Antibody (HL1645) - Azide and BSA Free [NBP3-25679] -

Immunohistochemistry-Paraffin: SETDB1 Antibody (HL1645) - Azide and BSA Free [NBP3-25679] - SETDB1 antibody [HL1645] detects SETDB1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. SETDB1 stained by SETDB1 antibody [HL1645] (NBP3-25679) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for SETDB1 Antibody (HL1645) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SETDB1

The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 039;Enhancer of zeste 039 proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. [supplied by OMIM]

Alternate Names

ERG-associated protein with SET domain, ESETEC 2.1.1.43, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase SETDB1, histone-lysine N-methyltransferase, H3lysine-9 specific 4, KG1T, KIAA0067H3-K9-HMTase4, KMT1EERG-associated protein with a SET domain, ESET, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1

Gene Symbol

SETDB1

Additional SETDB1 Products

Product Documents for SETDB1 Antibody (HL1645) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SETDB1 Antibody (HL1645) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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