SETDB1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20321
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Bovine (96%). Backed by our 100% Guarantee.
Applications
Validated:
Block/Neutralize, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Western Blot
Cited:
Block/Neutralize, In vitro assay
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human SETDB1. The exact sequence is proprietary.
Reactivity Notes
Rat (89%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
143 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SETDB1 Antibody
Chromatin Immunoprecipitation: SETDB1 Antibody [NBP2-20321] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-SETDB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to P53P2.
![Immunoprecipitation: SETDB1 Antibody [NBP2-20321]](https://resources.bio-techne.com/images/products/SETDB1-Antibody-Immunoprecipitation-NBP2-20321-img0003.jpg "Immunoprecipitation: SETDB1 Antibody [NBP2-20321]")
Immunoprecipitation: SETDB1 Antibody [NBP2-20321]
Immunoprecipitation: SETDB1 Antibody [NBP2-20321] - IP samples: HeLa nuclear extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N1], N-term 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N1], N-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent].
Western Blot: SETDB1 Antibody [NBP2-20321] -
Western Blot: SETDB1 Antibody [NBP2-20321] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.Applications for SETDB1 Antibody
Application
Recommended Usage
Block/Neutralize
Reported in scientific literature (PMID: 24844442)
Chromatin Immunoprecipitation (ChIP)
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Immunoprecipitation
1:100-1:500
In vitro assay
Reported in scientific literature (PMID: 24844442)
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SETDB1
Alternate Names
ERG-associated protein with SET domain, ESETEC 2.1.1.43, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase SETDB1, histone-lysine N-methyltransferase, H3lysine-9 specific 4, KG1T, KIAA0067H3-K9-HMTase4, KMT1EERG-associated protein with a SET domain, ESET, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1
Gene Symbol
SETDB1
UniProt
Additional SETDB1 Products
Product Documents for SETDB1 Antibody
Product Specific Notices for SETDB1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.