SETDB2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20323
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for SETDB2 Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human SETDB2. The exact sequence is proprietary.
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SETDB2 Antibody
![Western Blot: SETDB2 Antibody [NBP2-20323]](https://resources.bio-techne.com/images/products/SETDB2-Antibody-Western-Blot-NBP2-20323-img0001.jpg "Western Blot: SETDB2 Antibody [NBP2-20323]")
Western Blot: SETDB2 Antibody [NBP2-20323]
Western Blot: SETDB2 Antibody [NBP2-20323] - Sample (30 ug of whole cell lysate) A: SK-N-SH 7. 5% SDS PAGE gel, diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: SETDB2 Antibody [NBP2-20323]](https://resources.bio-techne.com/images/products/SETDB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20323-img0004.jpg "Immunocytochemistry/ Immunofluorescence: SETDB2 Antibody [NBP2-20323]")
Immunocytochemistry/ Immunofluorescence: SETDB2 Antibody [NBP2-20323]
Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NBP2-20323] - HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETDB2 protein stained by SETDB2 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.![Immunoprecipitation: SETDB2 Antibody [NBP2-20323]](https://resources.bio-techne.com/images/products/SETDB2-Antibody-Immunoprecipitation-NBP2-20323-img0003.jpg "Immunoprecipitation: SETDB2 Antibody [NBP2-20323]")
Immunoprecipitation: SETDB2 Antibody [NBP2-20323]
Immunoprecipitation: SETDB2 Antibody [NBP2-20323] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of SETDB2 protein by 2 ug of SETDB2 antibody 7.5% SDS-PAGE The immunoprecipitated SETDB2 protein was detected by SETDB2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Applications for SETDB2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SETDB2
Alternate Names
C13orf4, chromosome 13 open reading frame 4, chronic lymphocytic leukemia deletion region 8, Chronic lymphocytic leukemia deletion region gene 8 protein, CLLD8DKFZp761J1217, DKFZp586I0123, EC 2.1.1.43, histone-lysine N-methyltransferase SETDB2, KMT1FCLLL8, Lysine N-methyltransferase 1F, SET domain bifurcated 2, SET domain, bifurcated 2
Gene Symbol
SETDB2
Additional SETDB2 Products
Product Documents for SETDB2 Antibody
Product Specific Notices for SETDB2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.