Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SETMAR Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human SET Domain and Mariner Transposase Fusion Gene-Containing Protein using the numbering given in entry BAD96454.1 (GeneID 6419).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SETMAR Antibody

Immunoprecipitation: SETMAR Antibody [NBP1-97739]

Immunoprecipitation: SETMAR Antibody [NBP1-97739]

Immunoprecipitation: SETMAR Antibody [NBP1-97739] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug ) and Jurkat (J; 50 ug ) cells. Antibodies: Affinity purified rabbit anti-Metnase antibody NBP1-97739 used for WB at 0.4 ug /ml (A) and 1 ug /ml (B) and used for IP at 6 ug /mg lysate. Metnase was also immunoprecipitated by rabbit anti-Metnase antibody NBP1-97740 , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Applications for SETMAR Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SETMAR

Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3, 2 specific tags for epigenetic transcriptional activation. Specifically mediates dimethylation of H3 'Lys-36'. Has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. Has DNA nicking activity. Has in vivo end joining activity and may mediate genomic integration of foreign DNA

Alternate Names

EC 2.1.1.43, histone-lysine N-methyltransferase SETMAR, HsMar1, Metnase, SET domain and mariner transposase fusion gene, SET domain and mariner transposase fusion gene-containing protein

Gene Symbol

SETMAR

Additional SETMAR Products

Product Documents for SETMAR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SETMAR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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