SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/4368R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14291
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # NR5A1/4368R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment of human SF-1/NR5A1/Steroidogenic Factor 1 protein (exact sequence is proprietary) (Uniprot: Q13285)
Localization
Nuclear
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13917).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images
SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/4368R)
Formalin-fixed, paraffin-embedded human renal oncocytoma stained with SF-1/NR5A1/Steroidogenic Factor 1 antibody (NR5A1/4368R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/4368R)
Formalin-fixed, paraffin-embedded human adrenal cortical tumor stained with SF-1/NR5A1/Steroidogenic Factor 1 antibody (NR5A1/4368R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: SF-1/NR5A1
Long Name
Steroidogenic Factor 1
Alternate Names
AD4BP, FTZF1, NR5A1, SF1
Gene Symbol
NR5A1
Additional SF-1/NR5A1 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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