SF-1/NR5A1/Steroidogenic Factor 1 Antibody (SF1/8000R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20253

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SF1/8000R

Concentration

0.2 mg/ml

View all SF-1/NR5A1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa200-400) of human SF-1/NR5A1/Steroidogenic Factor 1 protein (exact sequence is proprietary)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Human testis or adrenal cortical carcinoma.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (SF1/8000R)

SF-1/NR5A1/Steroidogenic Factor 1 Antibody (SF1/8000R)

SF-1/NR5A1/Steroidogenic Factor 1 Antibody (SF1/8000R)

Formalin-fixed, paraffin-embedded human testis stained with SF-1/NR5A1/Steroidogenic Factor 1 antibody (SF1/8000R). Inset: PBS instead of primary antibody; secondary only negative control.
SF-1/NR5A1/Steroidogenic Factor 1 Antibody (SF1/8000R)

SF-1/NR5A1/Steroidogenic Factor 1 Antibody (SF1/8000R)

Formalin-fixed, paraffin-embedded human adrenal cortex stained with SF-1/NR5A1/Steroidogenic Factor 1 antibody (SF1/8000R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (SF1/8000R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SF-1/NR5A1

Steroidogenic factor 1 (SF-1), a NR5 Fushi Tarazu-Like Receptor, has been shown to affect sexual differentiation, steroid metabolism, and neural development. SF-1 binds as a monomer and regulates all steroidogenic p450 genes, Mullerian inhibiting substance, the gonadotrophins, the ACTH receptor, oxytocin, nuclear receptor DAX-1, and several other genes. Four alternatively spliced isoforms of SF-1 have been isolated in mice, and they differ at the N and C termini but are identical in the DNA-binding domain. SF-1 expression has been documented in human brain, adrenal, spleen, liver, heart, ovary, testis, and placenta. ESTs have been isolated from human tissue libraries, including cancerous adrenal and normal lung and testis.

Long Name

Steroidogenic Factor 1

Alternate Names

AD4BP, FTZF1, NR5A1, SF1

Gene Symbol

NR5A1

Additional SF-1/NR5A1 Products

Product Documents for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (SF1/8000R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (SF1/8000R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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