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SF2 Antibody (3J5Q9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16329

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16329-100ul
NBP3-16329-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3J5Q9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for SF2 Antibody (3J5Q9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SF2 (NP_008855.1). GGGGGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SF2 Antibody (3J5Q9)

Western Blot: SF2 Antibody (3J5Q9) [NBP3-16329]

Western Blot: SF2 Antibody (3J5Q9) [NBP3-16329]

Western Blot: SF2 Antibody (8L3T3) [NBP3-16329] - Western blot analysis of extracts of various cell lines, using SRSF1/SF2 Rabbit mAb (NBP3-16329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry-Paraffin: SF2 Antibody (3J5Q9) [NBP3-16329]

Immunohistochemistry-Paraffin: SF2 Antibody (3J5Q9) [NBP3-16329]

Immunohistochemistry-Paraffin: SF2 Antibody (8L3T3) [NBP3-16329] - Immunohistochemistry of paraffin-embedded mouse brain using SRSF1/SF2 Rabbit mAb (NBP3-16329) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: SF2 Antibody (3J5Q9) [NBP3-16329]

Immunohistochemistry-Paraffin: SF2 Antibody (3J5Q9) [NBP3-16329]

Immunohistochemistry-Paraffin: SF2 Antibody (8L3T3) [NBP3-16329] - Immunohistochemistry of paraffin-embedded rat brain using SRSF1/SF2 Rabbit mAb (NBP3-16329) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for SF2 Antibody (3J5Q9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SF2

SF2 encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Alternative-splicing factor 1, ASFpre-mRNA-splicing factor SF2, P33 subunit, MGC5228, serine/arginine-rich splicing factor 1, SF2FLJ53078, SF2p33, SFRS1splicing factor 2, Splicing factor, arginine/serine-rich 1ASF-1, SR splicing factor 1, SRp30a

Gene Symbol

SRSF1

Additional SF2 Products

Product Documents for SF2 Antibody (3J5Q9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SF2 Antibody (3J5Q9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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